• Product NameGJA9 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total GJA9 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 246-260 amino acids of Human gap junction protein, alpha 9, 59kDa
• Target NameGJA9
• Alternative Names    gap junction protein, alpha 9, 59kDa, CX58, CX59, GJA10
• Accession No. Swiss-Prot:P57773
  Gene ID:81025;
• UniprotP57773
• Gene ID81025;
• Concentration 0.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 59kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:2000-1:5000

Connexins, such as GJA9, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

• Gel: 8%SDS-PAGE
  Lane1: Human fetal muscle tissue lysate
  Lane2: OP9 cell lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 1 minute

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting