• Product NameEDNRB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total EDNRB protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide peptide corresponding to a region derived from 70-84 amino acids of human endothelin receptor type B
• Target NameEDNRB
• Alternative Names    endothelin receptor type B, ETB, ET-B, ETBR, ETRB, HSCR, WS4A, ABCDS, ET-BR, HSCR2
• Accession No. Swiss-Prot:P24530
  Gene ID:1910;
• UniprotP24530
• Gene ID1910;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 50kd
  ELISA: 1:2000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• Gel: 10% SDS-PAGE
  Lane1: Human fetal lung tissue lysate
  Lane2: Human fetal intestine tissue lysate
  Lane3: Human lung tissue lysate
  Lane4: Hela cell lysate
  Lysates: 40ug per lane
  Primary antibody: 1/500 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
  Exposure time: 1 minute
• The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 31191 (EDNRB Antibody) at dilution 1/30, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting