• Product NameSEK1/MKK4(Ab-80) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total SEK1/MKK4 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 78~82 (T-H-S-I-E) derived from Human SEK1/MKK4.
• Target NameSEK1/MKK4
• Alternative Names    JNKK; JNKK1; MAP2K4; MAPK/ERK kinase 4; kinase 4
• Accession No. Swiss-Prot: P45985
  NCBI Protein: NP_003001.1
• UniprotP45985
• Gene ID6416;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 44kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).

Park HS, et al. (2002) J Biol Chem; 277(4): 2573-8.

• Western blot analysis of extracts from PC12 and C2C12 cells using SEK1/MKK4(Ab-80) Antibody #21132.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SEK1/MKK4(Ab-80) Antibody #21132(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting