• Product NameNMDAR1(Ab-896) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total NMDAR1protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.894~898 (R-R-S-S-K) derived from Human NMDAR1.
• Target NameNMDAR1
• Alternative Names    GLURZ1; GRIN1; NMD-R1; NMDZ1; NMZ1
• Accession No. Swiss-Prot: Q05586
  NCBI Protein: NP_000823.4
• UniprotQ05586
• Gene ID2902;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 120kd
  Western blotting: 1:500~1:1000
   

NMDA receptors are members of the ionotropic class of glutamate receptors, which also includes Kainate and AMPA receptors. NMDA receptors consist of NR1 subunits combined with one or more NR2 (A-D) or NR3 (A-B) subunits. The ligand-gated channel is permeable to cations including Ca2+, and at resting membrane potentials NMDA receptors are inactive due to a voltage-dependent blockade of the channel pore by Mg2+. NMDA receptor activation, which requires binding of glutamate and glycine, leads to an influx of Ca2+ into the postsynaptic region where it activates several signaling cascades, including pathways leading to the induction of long-term potentiation (LTP) and depression (LTD). NMDA receptors have a critical role in excitatory synaptic transmission and plasticity in the CNS. They govern a range of physiological conditions including neurological disorders caused by excitotoxic neuronal injury, psychiatric disorders and neuropathic pain syndromes.

Tyszkiewicz JP, et al. J Physiol. 2004 Feb 1; 554(Pt 3): 765-777

• Western blot analysis of extracts from PC12 and C6 cells using NMDAR1(Ab-896) Antibody #21133.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting