• Product NameHCN1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total HCN1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 874-886 amino acids of Human hyperpolarization activated cyclic nucleotide-gated potassium channel 1
• Target NameHCN1
• Alternative Names    hyperpolarization activated cyclic nucleotide-gated potassium channel 1, BCNG1, HAC-2, BCNG-1
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 99kd
  ELISA: 1:2000-1:10000
  Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:50-1:200

The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes.

• Gel: 8%SDS-PAGE
  Lane1: Human brain malignant glioma tissue lysate
  Lane2: Mouse brain tissue lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/1500 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 2 minutes
  
• The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 31214 (HCN1 Antibody) at dilution 1/80, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting