• Product NameMMP27 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total MMP27 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 440-452 amino acids of Human matrix metallopeptidase 27
• Target NameMMP27
• Alternative Names    Matrix metallopeptidase 27, MMP-27
• Accession No. Swiss-Prot:Q9H306
  Gene ID:64066;
• UniprotQ9H306
• Gene ID64066;
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 59kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.

• Gel: 10%SDS-PAGE
  Lysate: 80 µg Mouse brain tissue lysate
  Primary antibody: 1/500 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 2 minutes
• The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using 31242 (MMP27 Antibody) at dilution 1/40, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting