• Product NameF2R Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total F2R protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 56-70 amino acids of human coagulation factor II (thrombin) receptor
• Target NameF2R
• Alternative Names    coagulation factor II (thrombin) receptor, TR, HTR, CF2R, PAR1, PAR-1
• Accession No. Swiss-Prot:P25116
  Gene ID:2149;
• UniprotP25116
• Gene ID2149;
• Concentration 0.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 47kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

• Gel: 10+12% SDS-PAGE
  Lane1: K562 cel lysate
  Lane2: Human lymphoma tissue lysate
  Lane3: Human fetal kidney tissue lysate
  Lysates: 40ug per lane
  Primary antibody: 1/650 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
  Exposure time: 2 minutes
  
• The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 31256 (F2R Antibody) at dilution 1/40, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting