• Product NamePDK4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total PDK4 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 101-113 amino acids of Human pyruvate dehydrogenase kinase, isozyme 4
• Target NamePDK4
• Alternative Names    Pyruvate dehydrogenase kinase, isozyme 4
• Accession No. Swiss-Prot:Q16654
  Gene ID:5166;
• UniprotQ16654
• Gene ID5166;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 46kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.

• Gel: 10%SDS-PAGE
  Lysate: 60 µg Mouse pancreas tissue lysate
  Primary antibody: 1/700 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 2 minutes
• The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using 31258 (PDK4 Antibody) at dilution 1/50, on the right is treated with the synthetic peptide.
• Immunohistochemistry of paraffin-embedded Human tonsil tissue using PDK4 antibody at dilution of 1:50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting