• Product NameGEMIN2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total GEMIN2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 264-280 amino acids of human gem (nuclear organelle) associated protein 2
• Target NameGEMIN2
• Alternative Names    gem (nuclear organelle) associated protein 2, SIP1, SIP1-delta
• Accession No. Swiss-Prot:O14893
  Gene ID:8487;
• UniprotO14893
• Gene ID8487;
• Concentration 0.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 32kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.

• Gel: 10%SDS-PAGE
  Lane1: K562 cell lysate
  Lane2: Hela cell lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 2 minutes
  
• The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using 31266 (GEMIN2 Antibody) at dilution 1/40, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting