• Product NameAKR1A1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total AKR1A1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 312-324 amino acids of human aldo-keto reductase family 1, member A1 (aldehyde reductase)
• Target NameAKR1A1
• Alternative Names    Aldo-keto reductase family 1, member A1 (aldehyde reductase), ALR; ARM; DD3; ALDR1
• Accession No. Swiss-Prot:P14550
  Gene ID:10327;
• UniprotP14550
• Gene ID10327;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 37kd
  ELISA: 1:2000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein.

• Gel: 10%SDS-PAGE
  Lane1: Human liver cancer tissue lysate
  Lane2: Hela cell lysate
  Lane3: 293T cell lysate
  Lysates: 27 ug per lane
  Primary antibody: 1/500 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 10 seconds
  
• The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using 31277 (AKR1A1 Antibody) at dilution 1/30, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting