• Product NameENO2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total ENO2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 420-434 amino acids of Human enolase 2 (gamma, neuronal)
• Target NameENO2
• Alternative Names    Enolase 2 (gamma, neuronal), NSE
• Accession No. Swiss-Prot:P09104
  Gene ID:2026;
• UniprotP09104
• Gene ID2026;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 47kd
  ELISA: 1:2000-1:10000
  Western blotting: 1:1000-1:5000

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.?Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

• Gel: 10%SDS-PAGE
  Lane1: 293T cell lysate
  Lane2: Jurkat cell lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/1250 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 30 seconds
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting