• Product NameCK12 Antibody FITC Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• Applications IF
• Species ReactivityHu Ms Rt
• Immunogen DescriptionKLH conjugated synthetic peptide derived from human CK12 Cytokeratin 12
• ConjugatesFITC
• Excitation Emission494nm 518nm
• Target NameCK12
• Alternative Names    65 kDa cytokeratin; CK 12; CK 3; CK12; CK3; Cytokeratin 12; Cytokeratin 3; K12; K3; keratin 12 Meesmann corneal dystrophy; Keratin 12; Keratin 3; Keratin, type I cytoskeletal 12; K1C12_HUMAN; Keratin, type II cytoskeletal 3; KRT12; KRT3.
• Accession No.
  NCBI Gene ID3859
• UniprotQ99456
• Gene ID3859;
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• IF=1:50-200

Cytokeratin 12 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with family member KRT12. Cytokeratin 12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Defects in KRT3 and KRT12 are a cause of Meesmann corneal dystrophy (MCD), an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Symptoms occur in adulthood and include rupture of the corneal microcysts that may lead to photophobia, contact lens intolerance and intermittent diminution of visual acuity. Defects in KRT12 are a cause of juvenile epithelial corneal dystrophy of Meesmann (MCD)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting