• Product NamePAK1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total PAK1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 225-238 amino acids of Human p21 protein (Cdc42/Rac)-activated kinase
• Target NamePAK1
• Alternative Names    P21 protein (Cdc42/Rac)-activated kinase 1, PAKalpha
• Accession No. Swiss-Prot:Q13153
  Gene ID:5058;
• UniprotQ13153
• Gene ID5058;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 61kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.?

• Gel: 10%SDS-PAGE
  Lysate: 40 µg 293T cell lysate
  Primary antibody: 1/700 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 1 minute
• The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using 31287 (PAK1 Antibody) at dilution 1/40, on the right is treated with the synthetic peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting