• Product NameACO2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total ACO2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 766-780 amino acids of human aconitase 2, mitochondrial
• Target NameACO2
• Alternative Names    Aconitase 2, mitochondrial, ICRD; ACONM
• Accession No. Swiss-Prot:Q99798
  Gene ID:50;
• UniprotQ99798
• Gene ID50;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 85kd
  ELISA: 1:2000-1:5000
  Western blotting: 1:500-1:2000

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.?

• Gel: 8%SDS-PAGE
  Lane1: Mouse heart tissue lysate
  Lane2: Hela cell lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 3 seconds
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting