• Product NameHSP27(Ab-15) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total HSP27 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.13~17 (G-P-S-W-D) derived from Human HSP27.
• Target NameHSP27
• Alternative Names    28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; HSP 27; HSP25
• Accession No. Swiss-Prot: P04792
  NCBI Protein: NP _001531.1
• UniprotP04792
• Gene ID3315;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 27kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Involved in stress resistance and actin organization.

• Western blot analysis of extracts from Hela cells using HSP27(Ab-15) Antibody #21144 and the same antibody preincubated with blocking peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Ab-15) Antibody #21144(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting