• Product NameHIF1A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationImmunogen affinity purified
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityHIF1a antibody detects endogenous levels of HIF1a.
• Immunogen TypePeptide
• Immunogen DescriptionA synthesized peptide derived from human HIF1a.
• Target NameHIF1A
• Alternative Names    HIF-1a,HIF1A
• Accession No. Swiss-Prot#: Q16665
• UniprotQ16665
• Gene ID3091;
• SDS-PAGE MW 120KD
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C/1 year

•  WB: 1:500-1:3000  
   IHC: 1:50-1:200

Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, Arnt 1 (also designated HIF-1β), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family.

• Western blot analysis on LOVO using HIF1a antibody ,The lane on the left is blocked with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting