• Product NameCDC2(Ab-161) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total CDC2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.159~163 (T-Y-T-H-E) derived from Human CDC2.
• Target NameCDC2
• Alternative Names    CDC28; CDC2A; CDK1; Cyclin-dependent kinase 1;
• Accession No. Swiss-Prot: P06493
  NCBI Protein: NP_001163877.1
• UniprotP06493
• Gene ID983;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 34kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6
Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53
Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56
Liu Y, et al.(2004) J Biol Chem; 279(6): 4507-14

• Western blot analysis of extracts from COLO cells using CDC2(Ab-161) Antibody #21152 and the same antibody preincubated with blocking peptide.
• Immunofluorescence staining of methanol-fixed Hela cells using CDC2(Ab-161) Antibody #21152.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting