• Product NameAtaxin 1 (Phospho-Ser776) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of Ataxin 1 only when phosphorylated at Serine 776.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 776 (R-W-S(p)-A-P) derived from Human Ataxin 1.
• Target NameAtaxin 1
• ModificationPhospho
• Alternative Names    ATX1, D6S504E, SCA1
• Accession No. Swiss-Prot#: P54253;
  NCBI Gene#: 6310;
  NCBI Protein#: NP_000323.2
• UniprotP54253
• Gene ID6310;
• SDS-PAGE MW 87kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 87kd
  Western blotting: 1:500~1:1000

Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function.

• Western blot analysis of extracts from HepG2 cells using Ataxin 1 (Phospho-Ser776) Antibody #12034.The lane on the left is treated with the antigen-specific peptide.
• Western blot analysis of lysates from HepG2 cells treated with Adriamycin 0.5uM 5h, using Ataxin 1 (Phospho-Ser776) Antibody. The lane on the right is blocked with the phospho peptide.
• Immunofluorescence analysis of NIH/3T3 cells, using Ataxin 1 (Phospho-Ser776) Antibody. The picture on the right is blocked with the phospho peptide.
• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting