• Product NameDDX58 (Phospho-Thr170) Antibody HRP Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsWB IHC-P IHC-F
• Species ReactivityHu Ms
• Immunogen DescriptionKLH conjugated synthetic phosphopeptide derived from human DDX58 around the phosphorylation site of Thr170
• ConjugatesHRP
• Excitation EmissionN A
• Target NameDDX58 Thr170
• Alternative Names    DEAD H Asp-Glu-Ala-Asp His box polypeptide RIG-I; DKFZp434J1111; DKFZp686N19181; FLJ13599; C330021E21; OTTHUMP00000045225; DEAD Asp-Glu-Ala-Asp box polypeptide 58; DEAD Asp Glu Ala Asp His box polypeptide; DEAD box protein 58; Probable ATP dependent RNA helicase DDX58; Retinoic acid inducible gene 1
• Accession No.
  NCBI Gene ID23586
• UniprotO95786
• Gene ID23586;
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200

The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG1 DDX58) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG1 DDX58 activates the kinases TBK1 and IKKe through the adaptor protein IPS1. These kinases then phosphorylate the transcription factors IRF3 and IRF7 which are essential for the expression of type-I interferons. RIG1 DDX58 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting