• Product NameTNNI3 (Phospho-Ser43) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of TNNI3 only when phosphorylated at Serine 43.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human TNNI3 around the phosphorylation site of Ser43.
• Target NameTNNI3
• ModificationPhospho
• Alternative Names    CMH7, RCM1, cTnI, CMD2A, TNNC1
• Accession No. Swiss-Prot#: P19429;
  NCBI Gene#: 7137;
  NCBI Protein#: NP_000354.4
• UniprotP19429
• Gene ID7137;
• SDS-PAGE MW 26kd
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20˚C/1 year

• Predicted MW: 26kd
  Western blotting: 1:500~1:1000

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008],

• Western blot analysis of lysates from Jurkat cells, using TNNI3 (Phospho-Ser43) Antibody. The lane on the right is blocked with the phospho peptide.
• Immunohistochemistry analysis of paraffin-embedded human heart, using TNNI3 (Phospho-Ser43) Antibody. The picture on the right is blocked with the phospho peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting