• Product NameNa,K-ATPase alpha1 (Phospho-Ser23) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityRt
• SpecificityThe antibody detects endogenous level of Na,K-ATPase alpha1 only when phosphorylated at Serine 23.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Serine 23 (K-K-S(p)-K-K) derived from Rat Na,K-ATPase alpha1.
• Target NameNa,K-ATPase alpha1
• ModificationPhospho
• Alternative Names    Nkaa1b
• Accession No. Swiss-Prot#: P05023;
  NCBI Gene#: 476;
  NCBI Protein#: NP_000692.2
• UniprotP05023
• Gene ID476;
• SDS-PAGE MW 113kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 113kd
  Western blotting: 1:500~1:1000

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

• Western blot analysis of extracts from Rat brain tissue using Na,K-ATPase alpha1(Phospho-Ser23) Antibody #12052.The lane on the left is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting