• Product NamePI3-kinase p85- alpha (Phospho-Tyr607) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB,IHC,IF,ELISA
• Species ReactivityHuman,Mouse,Rat,Chicken
• SpecificityThe antibody detects endogenous level of PI3-kinase p85- alpha only when phosphorylated at Tyrosine 607.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Tyrosine 607 (D-Q-Y(p)-S-L) derived from Human PI3-kinase p85-alpha.
• Target NamePI3-kinase p85- alpha
• ModificationPhospho
• Alternative Names    p85, AGM7, GRB1, p85-ALPHA, PIK3R1
• Accession No. Swiss-Prot#: P27986;
  NCBI Gene#: 5295;
  NCBI Protein#: NP_001229395.1
• UniprotP27986
• Gene ID5295;
• SDS-PAGE MW 80kd
• Concentration 0.6mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 80kd
  Western blotting: 1:200~1:500

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

• All Lanes: PI3-kinase p85- (Phospho-Tyr607) Antibody at 1/ 500 dilution.
  Lane1: Rat kidney tissue lysate
  Lane2: Rat kidney tissue lysate, Primary antibody blocked with the 1ug/mL phospho peptide at 37°c for 2h Lysates/ Proteins at 60ug per lane
  Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution，37°c 1h
  Predicted band size : 80kDa Observed band size: 80kDa
• Immunohistochemical analysis of paraffin-embedded Human placenta. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
• Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
• Immunohistochemistry analysis of paraffin-embedded human brain, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The picture on the right is blocked with the phospho peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting