• Product NameiNOS (Phospho-Tyr151) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB, IH, ELISA
• Species ReactivityHu Rt
• SpecificityThe antibody detects endogenous level of iNOS only when phosphorylated at Tyrosine 151.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of Tyrosine 151 (Q-Y-Y(p)-G-S) derived from Human iNOS.
• Target NameiNOS
• ModificationPhospho
• Alternative Names    NOS, INOS, NOS2A, HEP-NOS
• Accession No. Swiss-Prot#: P35228;
  NCBI Gene#: 4843;
  NCBI Protein#: NP_000616.3
• UniprotP35228
• Gene ID4843;
• SDS-PAGE MW 130kd
• Concentration 1.0mg/ml
• Formulation Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Storage Store at -20˚C/1 year

• WB (1/500 - 1/1000), IH (1/50 - 1/100)

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

• Western blot analysis of iNOS (pY151) expression in HeLa (A), Jurkat (B), MCF7 (C) whole cell lysates.
• Immunohistochemical analysis of iNOS (pY151) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
• Direct ELISA antibody dose-response curve using Anti-iNOS (pY151) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting