• Product NameHistone H3(Acetyl-Lys9) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic acetylpeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific acetylpeptide. Non-acetyl specific antibodies were removed by chromatogramphy using non-acetylpeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Histone H3 only when acetylated at Lysine 9.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around acetylation site of Lysine 9(T-A-R(p)-K-S) derived from Human Histone H3.
• Target NameHistone H3
• ModificationAcetyl
• Alternative Names    H3/A, H3FA
• Accession No. Swiss-Prot#: P68431;
  NCBI Gene#: 8350;
  NCBI Protein#: NP_003529.1
• UniprotP68431
• Gene ID8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
• SDS-PAGE MW 17kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 17kd
  Western blotting: 1:500~1:1000

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

• Western blot analysis of extracts from Hela cells treated with TSA using Histone H3(Acetyl-Lys9) Antibody #12069.The lane on the left is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting