• Product NameSrc(Ab-529) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Src protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 527~531 (P-Q-Y-Q-P) derived from Human Src.
• Target NameSrc
• Alternative Names    C-SRC; SRC1;
• Accession No. Swiss-Prot: P12931
  NCBI Protein: NP_005408.1
• UniprotP12931
• Gene ID6714;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 60kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.

Pyper J.M., (1985) Mol. Cell. Biol. 5:831-838
Pyper J.M.(1990) Mol. Cell. Biol. 10:2035-2040
Xu W., (1997).Nature 385:595-602
Benes C.H., (2005) Cell 121:271-280

• Western blot analysis of extracts from HT29, Hela and MDA cells using Src(Ab-529) Antibody #21168.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src(Ab-529) Antibody #21168(left) or the same antibody preincubated with blocking peptide(right).
• Western Blot analysis of Hela /293 cells using Src Polyclonal Antibody

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting