• Product NamePAK1/PAK2/PAK3(Ab-423/402/421) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total PAK1/PAK2/PAK3 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.421~425/400~404/419~423 (R-S-T-M-V) derived from Human PAK1/PAK2/PAK3.
• Target NamePAK1/PAK2/PAK3
• Alternative Names    p21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK;
• Accession No. Swiss-Prot:Q13153
  Gene ID:5058
• UniprotQ13153
• Gene ID5058;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 61-67 (PAK2) 68-74 (PAK1/3)kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Parsons M, et al. (2005) Mol Cell Biol ; 25:1680-1695.
Jung JH, et al. (2005) J Biol Chem; 280:40025-40031.
Lampugnani MG, et al. (2002) Mol Biol Cell ; 13: 1175-1189
Wang RA, et al. (2002) EMBO J; 21: 5437 - 5447.

• Western blot analysis of extracts from MCF-7 and Hela cells using PAK1/PAK2/PAK3(Ab-423/402/421) Antibody #21169.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3(Ab-423/402/421) Antibody #21169(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using PAK1/PAK2/PAK3(Ab-423/402/421) Antibody #21169.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting