• Product NameZap-70(Ab-493) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Zap-70 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.491~495 (S-Y-Y-T-A) derived from Human ZAP70.
• Target NameZap-70
• Alternative Names    70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70
• Accession No. Swiss-Prot: P43403
  NCBI Protein: NP _001070.2
• UniprotP43403
• Gene ID7535;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 70kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions

Chan AC, et al. (1995) EMBO J; 14(11): 2499-2508
Kong G, et al. (1996) Mol Cell Biol; 16(9): 5026-5035
Salomon AR, et al. (2003)Proc Natl Acad Sci U S A; 100(2): 443-448

• Western blot analysis of extracts from HL60 cells using Zap-70(Ab-493) Antibody #21174.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Zap-70(Ab-493) Antibody #21174(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting