• Product NameCHD3 (C-terminus) Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.7F6-G2-E5
• IsotypeIgG1
• ApplicationsWB ICC
• Species ReactivityHu Ms Rt
• SpecificityThis antibody detects endogenous levels of CHD3 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human CHD3 protein fragments expressed in E.coli.
• Target NameCHD3
• Alternative Names    ZFH; Mi-2a; Mi2-ALPHA
• Accession No. Uniprot: Q12873

  Gene ID: 1107

• UniprotQ12873
• Gene ID1107;
• SDS-PAGE MW 260kd
• Formulation ascites
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:400

 Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.

• Immunocytochemistry staining of HeLa cells fixed with -20℃ Methanol and using anti-CHD3 antibody (dilution 1:400).
• Western blot detection of CHD3 in NTERA2,3T3,C6,K562 and Hela cell lysates using CHD3 antibody (1:1000 diluted).Predicted band size: 260KDa.Observed band size: 260KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting