• Product NameCD54(ICAM-1) Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.4F5-F9-F12
• IsotypeIgG2b
• PurificationAffinity purified
• ApplicationsWB ICC
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of ICAM1(C-terminus) and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human ICAM1(C-terminus) protein fragments expressed in E.coli.
• Target NameCD54(ICAM-1)
• Alternative Names    BB 2; BB2; CD 54; CD_antigen=CD54; CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM 1; ICAM-1; ICAM1; ICAM1_HUMAN; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA 2; MALA2; MyD 10; MyD10; P3.58
• Accession No. Uniprot: P05362

  Gene ID: 3383

• UniprotP05362
• Gene ID3383;
• SDS-PAGE MW 96kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:200

 ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

• Immunocytochemistry staining of SW480 cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-ICAM1 antibody (dilution 1:200).
• Western blot detection of ICAM1 antibody in SW480 and Raji cell lysates using ICAM1 antibody (1:1000 diluted).Predicted band size:96KDa.Observed band size:96KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting