• Product NameGRB2 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.3B2-G2-E5
• IsotypeIgG1
• ApplicationsWB
• Species ReactivityHu Rt Ms Mk
• SpecificityThis antibody detects endogenous levels of GRB2 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human GRB2 protein fragments expressed in E.coli.
• Target NameGRB2
• Alternative Names    Abundant SRC homology; Adapter protein GRB2; ASH; Ash protein; EGFRBP GRB2; Epidermal growth factor receptor binding protein; Epidermal growth factor receptor binding protein GRB2; GRB 2; GRB2 adapter protein; Grb2; GRB2_HUMAN; Grb3 3;
• Accession No. Uniprot: P62993

  Gene ID: 2885

• UniprotP62993
• Gene ID2885;
• SDS-PAGE MW 25kd
• Formulation Ascites
• Storage store at -20˚C

•  Western blotting: 1:500

 Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

• Western blot detection of GRB2 in Jurkat,Raji,PC-12&3T3 cell lysates and using GRB2 antibody(1:500 diluted).Predicted band size: 25KDa Observed band size: 25KDa
• Western blot detection of GRB2 in MCF7,3T3,COS7,C6 and Hela cell lysates and using GRB2 antibody(1:300 diluted).Predicted band size: 25KDa.Observed band size: 25KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting