• Product NameCSK Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.2B4-C6-A10
• IsotypeIgG2b
• PurificationAffinity purified
• ApplicationsWB ICC
• Species ReactivityHu Rt
• SpecificityThis antibody detects endogenous levels of CSK and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human CSK protein fragments expressed in E.coli.
• Target NameCSK
• Alternative Names    C SRC; C SRC kinase; C src Tyrosine Kinase; C-SRC kinase; c-src tyrosine kinase; Csk A; CSK; CSK_HUMAN; CYTOPLASMIC TYROSINE KINASE; EC2.7.10.2; MGC112926; MGC117393; MGC154049; P60 Src; Protein tyrosine kinase CYL; Protein-tyrosine kinase CYL;
• Accession No. Uniprot: P41240

  Gene ID: 1445

• UniprotP41240
• Gene ID1445;
• SDS-PAGE MW 50kd
• Formulation Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:50

 Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.

• Immunocytochemistry staining of C6 cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-CSK antibody (dilution 1:50).
• Western blot detection of CSK antibody in C6 and Jurkat cell lysates using CSK antibody (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting