• Product NameDNA-PKcs Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.8D3-B12-F11
• IsotypelgG2b
• PurificationAffinity purified
• ApplicationsWB IP ICC
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of DNA-PKcs,and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human DNA-PKcs protein fragments expressed in E.coli
• Target NameDNA-PKcs
• Alternative Names    DNA dependent protein kinase catalytic subuni; DNA PKcs; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNAPK; DNPK1; hyper radiosensitivity of murine scid mutation, complementing 1; HYRC; HYRC1; p350; p460; PRKDC;
• Accession No. Uniprot: P78527

  Gene ID: 5591

• UniprotP78527
• Gene ID5591;
• SDS-PAGE MW 450kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:200

 properties.Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis(DCLRE1C).The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step.Required to protect and align broken ends of DNA.May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.Found at the ends of chromosomes,suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion.Also involved in modulation of transcription.Recognizes the substrate consensus sequence[ST]-Q.Phosphorylates'Ser-139' of histone variant H2AX/H2AFX,thereby regulating DNA damage response mechanism.Phosphorylates DCLRE1C,c-Abl/ABL1,histone H1,HSPCA,c-jun/JUN,p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2.Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA.Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.

• Immunocytochemistry stain of Hela using PRKDC antibody (1:200).
• Immunoprecipitation analysis of Hela cell lysates using PRKDC antibody
• Western blot detection of PRKDC in Hela,K562,HL-60&MOLT-4 cell lysates using PRKDC antibody(1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa
• Western blot detection of PRKDC in K562,Molt-4,A549 and HCT116 cell lysates using PRKDC antibody(1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting