• Product NameSMARCC1 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.2A4-H7-C12
• IsotypeIgG1
• PurificationAffinity purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of BAF155 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human BAF155 protein fragments expressed in E.coli.
• Target NameSMARCC1
• Alternative Names    AI115498; BAF 155; BAF155; BRG 1 associated factor 155; BRG1 associated factor 155; BRG1-associated factor 155; Chromatin remodeling complex BAF155 subunit; CRACC 1; CRACC1; Mammalian chromatin remodeling complex BRG 1 associated factor 155;
• Accession No. Uniprot: Q92922

  Gene ID: 6599

• UniprotQ92922
• Gene ID6599;
• SDS-PAGE MW 155
• Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Storage store at -20˚C

•  Western blotting: 1:1000

 Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).May stimulate the ATPase activity of the catalytic subunit of the complex.Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex,a chromatin-remodeling complex recruited by vitamin D receptor (VDR),which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex).During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state.The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes.As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A,are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF).The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells.The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth by similarity.

• Western blot detection of BAF155 antibody in K562,Jurkat and Hela cell lysates using BAF155 antibody (1:1000 diluted).Predicted band size:155KDa.Observed band size:155KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting