• Product NameUCHL1/PGP9.5 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.7B8-G5-E7
• IsotypeIgG2b
• PurificationAffinity purified
• ApplicationsWB ICC
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of UCHL1 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human UCHL1. protein fragments expressed in E.coli.
• Target NameUCHL1/PGP9.5
• Alternative Names    Neuron cytoplasmic protein 9.5; PGP 9.5; Ubiquitin thioesterase L1
• Accession No. Uniprot: P09936

  Gene ID: 7345

• UniprotP09936
• Gene ID7345;
• SDS-PAGE MW 25kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.2% sodium azide, 50% glycerol, 0.1%BSA
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:300

 Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity

• Immunocytochemistry stain of COS7 using UCHL1 antibody (1:300).
• Western blot detection of UCHL1 in U251,Raji&IMP32 cell lysates and using UCHL1 antibody (1:1000 diluted). Predicted band size: 25KDa Observed band size:25KDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting