• Product NamePlacental alkaline phosphatase (PLAP) Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.8F10-2C10-C9
• IsotypeIgG2b
• PurificationAffinity purified
• ApplicationsWB ICC
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of ALPP and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human ALPP protein fragments expressed in E.coli.
• Target NamePlacental alkaline phosphatase(PLAP)
• Alternative Names    Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1;
• Accession No. Uniprot: P05187

  Gene ID: 250

• UniprotP05187
• Gene ID250;
• SDS-PAGE MW 76kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.2% sodium azide, 50% glycerol, 0.1%BSA
• Storage store at -20˚C

• Western blotting: 1:500
  Immunocytochemistry: 1:300

 There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.

• Immunocytochemistry stain of Hela using ALPP antibody (1:300).
• Western blot detection of ALPP in Hela,LNCap,human Placenta &A549 cell lysates and using ALPP antibody (1:500 diluted). Predicted band size: 76KDa Observed band size:76KDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting