• Product NameRAD9A Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.3A3-A7-F8
• IsotypeIgG2b
• PurificationAffinity purified
• ApplicationsWB IP
• Species ReactivityHu Ms Rt Mk
• SpecificityThis antibody detects endogenous levels of RAD9A,and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human Rad9A protein fragments expressed in E.coli
• Target NameRAD9A
• Alternative Names    Cell cycle checkpoint control protein; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; RAD9A;
• Accession No. Uniprot: Q99638

  Gene ID: 5883

• UniprotQ99638
• Gene ID5883;
• SDS-PAGE MW 55kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.
• Storage store at -20˚C

•  Western blotting: 1:500

 Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair.The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC)clamp loader complex.Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER).The 9-1-1 complex stimulates DNA polymerase beta(POLB)activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds;endonuclease FEN1 cleavage activity on substrates with double,nick,or gap flaps of distinct sequences and lengths;and DNA ligase I(LIG1)on long-patch base excision repair substrates.The 9-1-1 complex is necessary for the recruitment of C12orf32/RHINO to sites of double-stranded breaks(DSB)occurring during the S phase.RAD9A possesses 3'->5' double stranded DNA exonuclease activity.Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

• Western blot detection of RAD9A in Hela,MCF7,3T3,COS7&Rat testis cell lysates using RAD9A antibody(1:500 diluted).Predicted band size:43KDa,Observed band size:55KDa
• Immunoprecipitation analysis of Hela & 3T3 cell lysates using RAD9A antibody

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting