• Product NameCHD4 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.5F1-A11-C9
• IsotypeIgG2b
• PurificationAffinity purified
• ApplicationsWB IP
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of CHD4 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human CHD4 protein fragments expressed in E.coli.
• Target NameCHD4
• Alternative Names    ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta;
• Accession No. Uniprot: Q14839

  Gene ID: 1108

• UniprotQ14839
• Gene ID1108;
• SDS-PAGE MW 260kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.
• Storage store at -20˚C

•  Western blotting: 1:1000

 Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.

• Western blot detection of CHD4 in K562,HelaNE,HL-60,MOLT-4,Jurkat & 293 cell lysates using CHD4 antibody(1:1000 diluted).Predicted band size:260KDa,Observed band size:260KDa.
• Immunoprecipitation analysis of K562 cell lysates using CHD4 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting