• Product NameAMPK beta 1 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.1A7-E11-E9
• IsotypeHu Ms Rt Mk
• PurificationAffinity purified
• ApplicationsWB ICC IP IHC
• Species ReactivityHu Ms Rt Mk
• SpecificityThis antibody detects endogenous levels of AMPK beta 1 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human AMPK beta 1 protein fragments expressed in E.coli.
• Target NameAMPK beta 1
• Alternative Names    1300015D22Rik; 5 AMP activated protein kinase subunit beta 1; 5''-AMP-activated protein kinase subunit beta-1; AAKB1_HUMAN; AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1; AMP-activated, noncatalytic, beta-1; AMPK; AMPK beta 1 chain;
• Accession No. Uniprot: Q9Y478

  Gene ID: 5564

• UniprotQ9Y478
• Gene ID5564;
• SDS-PAGE MW 38kd
• Formulation Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:100

 Non-catalytic subunit of AMP-activated protein kinase (AMPK),an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism.In response to reduction of intracellular ATP levels,AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein,carbohydrate and lipid biosynthesis,as well as cell growth and proliferation.AMPK acts via direct phosphorylation of metabolic enzymes,and by longer-term effects via phosphorylation of transcription regulators.Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton;probably by indirectly activating myosin.Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles,via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1,PRKAG2 or PRKAG3).

• Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using anti-AMPK beta 1 antibody (dilution 1:100).
• Western blot detection of AMPK beta 1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPK beta 1 antibody (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.
• Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 antibody.
• Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 Mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting