• Product NameDDB1 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.8E10-B8-E9
• IsotypeIgG2b
• ApplicationsWB
• Species ReactivityHu Ms Rt Mk
• SpecificityThis antibody detects endogenous levels of DDB1 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human DDB1 protein fragments expressed in E.coli.
• Target NameDDB1
• Alternative Names    Damage specific DNA binding protein 1; Damage-specific DNA-binding protein 1; DDB 1; DDB p127 subunit; Ddb1; DDB1_HUMAN; DDBa; DNA damage binding protein 1; DNA damage-binding protein 1; DNA damage-binding protein a; HBV X-associated protein 1;
• Accession No. Uniprot: Q16531

  Gene ID: 1642

• UniprotQ16531
• Gene ID1642;
• SDS-PAGE MW 127kd
• Formulation Ascites
• Storage store at -20˚C

•  Western blotting: 1:1500

 Required for DNA repair.Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex).The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair.The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD),6-4 photoproducts (6-4 PP),apurinic sites and short mismatches.Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins.The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1.DCX(DDB2) (also known as DDB1-CUL4-ROC1,CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A,histone H3 and histone H4 at sites of UV-induced DNA damage.The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair.DCX(DDB2) also ubiquitinates XPC,which may enhance DNA-binding by XPC and promote NER.DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication.DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR).May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.

• Western blot detection of DDB1 antibody in C6,COS7,293T,Raji and 3T3 cell lysates using DDB1 antibody (1:1500 diluted).Predicted band size:127KDa.Observed band size:127KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting