• Product NamePCNA Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.2E1-G10-H10
• IsotypeIgG2b
• ApplicationsWB IP ICC IHC
• Species ReactivityHu Ms Mk Rt Hm
• SpecificityThis antibody detects endogenous levels of PCNA and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human PCNA protein fragments expressed in E.coli.
• Target NamePCNA
• Alternative Names    Cyclin; Cyclin; DNA polymerase delta auxiliary protein; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; PCNA; Pcna/cyclin; PCNA_HUMAN; PCNAR;
• Accession No. Uniprot: P12004

  Gene ID: 5111

• UniprotP12004
• Gene ID5111;
• SDS-PAGE MW 36kd
• Formulation ascites
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:100

 Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities.Has to be loaded onto DNA in order to be able to stimulate APEX2.Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair:Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases,while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

• Immunocytochemistry staining of HeLa cells using anti-PCNA antibody (dilution 1:100).Fixed in 100% methanol for 2hr at -20℃.
• Western blot detection of PCNA antibody in Hela,3T3,COS7,C6 and CHO-K1 cell lysates using PCNA antibody (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.
• Immunoprecipitation analysis of Hela cell lysates using PCNA antibody.
• Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with PCNA Mouse mAb (2E1-G10-H10,1:400 diluted),showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting