• Product NameRPA70 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.8C3-D12-H10
• IsotypeIgG2a
• PurificationAffinity purified
• ApplicationsWB ICC IP
• Species ReactivityHu Mk Ms Rt
• SpecificityThis antibody detects endogenous levels of RPA70 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human RPA70 protein fragments expressed in E.coli.
• Target NameRPA70
• Alternative Names    Dmrpa1; Drosophila Replication Protein A; DRPA; HSSB; Human single stranded DNA binding protein; MST075; MSTP075; p70; REPA1; Replication factor A; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit;
• Accession No. Uniprot: P27694

  Gene ID: 6117

• UniprotP27694
• Gene ID6117;
• SDS-PAGE MW 70kd
• Formulation Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:100

 Plays an essential role in several cellular processes in DNA metabolism including replication,recombination and DNA repair.Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.Functions as component of the alternative replication protein A complex (aRPA).aRPA binds single-stranded DNA and probably plays a role in DNA repair;it does not support chromosomal DNA replication and cell cycle progression through S-phase.In vitro,aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC),the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

• Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using anti-RPA70 antibody (dilution 1:100).
• Western blot detection of RPA70 in Hela,A431,MCF7,COS7,HT1080 and K562 cell lysates using RPA70 antibody (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
• Western blot detection of RPA70 antibody in Hela,293T,C6,3T3 and K562 cell lysates using RPA70 antibody (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
• Western blot detection of RPA70 antibody in HUVEC,Hacat,3T3,MCF7 and A549 cell lysates using RPA70 antibody (1:2000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
• Immunoprecipitation analysis of Hela cell lysates using RPA70 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting