• Product NameWDR77 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.8A10-C10-E8
• IsotypeIgG1
• PurificationAffinity purified
• ApplicationsWB ICC
• Species ReactivityHu Ms Rt
• SpecificityThis antibody detects endogenous levels of WDR77 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human WDR77 protein fragments expressed in E.coli.
• Target NameWDR77
• Alternative Names    2610312E17Rik; Androgen receptor cofactor p44; C79984; HKMT1069; MEP 50; MEP-50; MEP50; MEP50_HUMAN; Methylosome protein 50; MGC2722; Nbla10071; p44; p44/Mep50; RGD1310479; RP11 552M11.3; WD repeat containing protein 77; WD repeat domain 77;
• Accession No. Uniprot: Q9BQA1

  Gene ID: 79084

• UniprotQ9BQA1
• Gene ID79084;
• SDS-PAGE MW 42kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:100

 Non-catalytic component of the 20S PRMT5-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones.This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles.Might play a role in transcription regulation.The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4),methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

• Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100，next using anti-WDR77 antibody (dilution 1:100).
• Western blot detection of WDR77 antibody in C6,3T3 and K562 cell lysates using WDR77 antibody (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting