• Product NameMLH1 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.2B3-H6-C11
• IsotypeIgG2b
• PurificationAffinity purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of MLH1 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human MLH1 protein fragments expressed in E.coli.
• Target NameMLH1
• Alternative Names    COCA 2; COCA2; DNA mismatch repair protein Mlh1; FCC 2; FCC2; hMLH 1; hMLH1; HNPCC 2; HNPCC; HNPCC2; MGC5172; MLH 1; MLH1; MLH1_HUMAN; MutL homolog 1 (E. coli); MutL homolog 1; MutL homolog 1 colon cancer nonpolyposis type 2;
• Accession No. Uniprot: P40692

  Gene ID: 4292

• UniprotP40692
• Gene ID4292;
• SDS-PAGE MW 85kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
• Storage store at -20˚C

•  Western blotting: 1:500

 Heterodimerizes with PMS2 to form MutL alpha,a component of the post-replicative DNA mismatch repair system (MMR).DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch,then MutL alpha is recruited to the heteroduplex.Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch.DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III,suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR.Also implicated in DNA damage signaling,a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

• Western blot detection of MLH1 antibody in Hela,293T and Jurkat cell lysates using MLH1 antibody (1:500 diluted).Predicted band size:85KDa.Observed band size:85KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting