• Product Namec-Fos Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.7D6-A9-D4
• IsotypeIgG1
• PurificationAffinity purified
• ApplicationsWB
• Species ReactivityHu Rt Mk
• SpecificityThis antibody detects endogenous levels of FOS and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human FOS protein fragments expressed in E.coli.
• Target Namec-Fos
• Alternative Names    Activator protein 1; AP 1; Cellular oncogene c fos; Cellular oncogene fos; FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral v fos oncogene homolog; FBJ Osteosarcoma Virus; FOS; FOS protein; FOS_HUMAN;
• Accession No. Uniprot: P01100

  Gene ID: 2353

• UniprotP01100
• Gene ID2353;
• SDS-PAGE MW 62kd
• Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:500

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor.In the heterodimer,FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites.On TGF-beta activation,forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling.Has a critical function in regulating the development of cells destined to form and maintain the skeleton.It is thought to have an important role in signal transduction,cell proliferation and differentiation.In growing cells,activates phospholipid synthesis,possibly by activating CDS1 and PI4K2A.This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor.In the heterodimer,FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites.On TGF-beta activation,forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling.Has a critical function in regulating the development of cells destined to form and maintain the skeleton.It is thought to have an important role in signal transduction,cell proliferation and differentiation.In growing cells,activates phospholipid synthesis,possibly by activating CDS1 and PI4K2A.This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

• Western blot detection of c-Fos antibody in HCT116,COS7 and C6 cell lysates using c-Fos antibody (1：500 diluted).Predicted band size:62KDa.Observed band size:62KDa.
• Western blot detection of c-Fos antibody in Hela(TSA treated ) and Hela cell lysates using c-Fos antibody (1:500 diluted).Predicted band size:62KDa.Observed band size:62KDa.
• Western blot analysis of extracts from Hela cells,untreated,50 μM TSA-treated or 50 μM TPA-treated for 24h,using C-FOS antibody (1:500 diluted)(upper) or GAPDH antibody (1:10000 diluted) (lower).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting