• Product NameBRAP Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.1E7-C9-D10-B10
• IsotypeIgG1
• PurificationAffinity purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThis antibody detects endogenous levels of BRAP and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human BRAP protein fragments expressed in E.coli.
• Target NameBRAP
• Alternative Names    3010002G07Rik; BRAP2; BRCA1 associated protein; EC 6.3.2.; Galectin 2 binding protein; IMP; Impedes mitogenic signal propagation; Renal carcinoma antigen NY REN 63; RING finger protein 52; RNF52; zgc:92894.
• Accession No. Uniprot: Q7Z569

  Gene ID: 8315

• UniprotQ7Z569
• Gene ID8315;
• SDS-PAGE MW 67kd
• Formulation Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:1000

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.

• Western blot detection of BRAP in A549 and HUVEC cell lysates using BRAP antibody (1:500 diluted).Predicted band size:67KDa.Observed band size:67KDa.
• Western blot analysis of extracts from CHO-K1 cells, transfected with a human pFLAG-CMV2-BRAP construct (A) or transfected with a human pFLAG-CMV2 construct (B), using BRAP antibody (1:1000 diluted).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting