• Product NamehHR23b Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.5H1-A10-A7
• IsotypeIgG2b
• PurificationAffinity purified
• ApplicationsWB ICC IHC
• Species ReactivityHu Ms Rt Mk Hm
• SpecificityThis antibody detects endogenous levels of hHR23b and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human hHR23b protein fragments expressed in E.coli.
• Target NamehHR23b
• Alternative Names    homolog of B; RAD23B; RD23B_HUMAN; UV excision repair protein RAD23 homolog B; XP C repair complementing complex 58 kDa; XP C repair complementing complex 58 kDa protein; XP C repair complementing protein; XP-C repair-complementing complex 58 kDa protein;
• Accession No. Uniprot: P54727

  Gene ID: 5887

• UniprotP54727
• Gene ID5887;
• SDS-PAGE MW 58kd
• Formulation Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:100

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome.
 

• Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-hHR23b antibody (dilution 1:100).
• Western blot detection of hHR23b in A431,K562,Jurkat,C6,3T3 and Hela cell lysates using hHR23b antibody (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.
• Immunohistochemical analysis of paraffin-embedded Prostate Cancer using hHR23b Mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
• Western blot detection of hHR23b in K562,SW480,CHO-K1,3T3 and COS7 cell lysates using hHR23b antibody (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting