• Product NameKu70 Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.7B1-E12-G4
• IsotypeIgG1
• PurificationAffinity purified
• ApplicationsWB ICC IP
• Species ReactivityHu Mk
• SpecificityThis antibody detects endogenous levels of Ku70 and does not cross-react with related proteins.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionPurified recombinant human Ku70 protein fragments expressed in E.coli.
• Target NameKu70
• Alternative Names    5''-deoxyribose-5-phosphate lyase Ku70; 5''-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1; ATP dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase 2 subunit 1;
• Accession No. Uniprot: P12956

  Gene ID: 2547

• UniprotP12956
• Gene ID2547;
• SDS-PAGE MW 67kd
• Formulation Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Storage store at -20˚C

• Western blotting: 1:1000

  Immunocytochemistry: 1:200

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.

• Immunocytochemistry staining of HeLa cells fixed with -20℃ Methanol and using anti-Ku70 antibody (dilution 1:200).
• Immunoprecipitation analysis of Hela cell lysates using Ku70 antibody.
• Western blot detection of Ku70 in Hela,A549,COS7 and K562 cell lysates using Ku70 antibody (1:1000 diluted).Predicted band size:70KDa.Observed band size:67KDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting