• Product NamePHB Antibody
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total PHB protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.256~260 (N-I-T-Y-L) derived from Human PHB.
• Alternative Names    Prohibitin; PHB1
• Accession No. Swiss-Prot: P35232
  NCBI Protein: NP_001268425.1
• UniprotP35232
• Gene ID5245;
• SDS-PAGE MW 29kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C

• Western blotting: 1:500～1:1000

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. 

• Western blot analysis of extracts from 3T3,Hela,MCF,PC12 and 293 cells using PHB Antibody  #21693.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting