• Product NameMAP3K7 (Phospho-Thr187) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of IMAP3K7 only when phosphorylated at threonine 187.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 187 (H-M-T(p)-N-N) derived from Human MAP3K7.
• Target NameMAP3K7
• ModificationPhospho
• Alternative Names    M3K7; MAP3K7; Mitogen-activated protein kinase kinase kinase 7; TGF-beta- activated kinase 1; kinase TAK1
• Accession No. Swiss-Prot#: O43318;
  NCBI Gene#: 6885;
  NCBI Protein#: NP_003179.1
• UniprotO43318
• Gene ID6885;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C/1 year

• Western blotting: 1:500~1:1000

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7.

Shin MS, et al. (2009)Biochim Biophys Acta 1793, 1156-64
Liu Q, Busby JC, Molkentin JD (2009)Nat Cell Biol 11, 154-61
Kim SI, Kwak JH, Wang L, Choi ME (2008)J Biol Chem 283, 10753-63

• Western blot analysis of extracts from NIH-3T3 tissue using MAP3K7 (Phospho-Thr187) antibody #11899.The lane on the right is treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting