• Product NamePKCth(Ab-695) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total PKCth protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.693~697 (N-F-S-F-M) derived from Human PKCth.
• Target NamePKCth
• Alternative Names    KPCT; PKC-theta; PKCQ; PRKCQ; PRKCT
• Accession No. Swiss-Prot: Q04759
  NCBI Protein: NP_006248.1
• UniprotQ04759
• Gene ID5588;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 80kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Xu ZB, et al.(2004) J Biol Chem 279:50401-50409
Thebault S, et al. (2004) Mol Immunol 40: 931-942

• Western blot analysis of extracts from HT29 cells using PKCth(Ab-695) Antibody #21185 and the same antibody preincubated with blocking peptide.
• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Ab-695) Antibody #21185(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting